abstract：:Albumin is the major circulating protein. It plays a fundamental role maintaining intra-vascular oncotic pressure and carrying many endogenous and exogenous substances. Variations of plasma albumin levels can be physiologic or pathologic and both qualitative and quantitative (more frequent) disorders are regrouped und...

journal_title：Annales de biologie clinique

authors： Bach-Ngohou K,Schmitt S,Le Carrer D,Masson D,Denis M

更新日期：2005-03-01 00:00:00

abstract：:The purpose of this study was to evaluate the cut-off value of brain natriuretic peptide (BNP) assayed with AxSYM BNP assay (Abbott). 86 patients have been included (mean age of 66 years). The clinical sensibility was 100% at 100 ng/L versus 80% at 250 ng/L. The clinical specificity was 66% at 100 ng/L versus 92% at 2...

journal_title：Annales de biologie clinique

authors： Ababsa R,Jourdain P,Funck F,Deschamps P,Sadeg N

更新日期：2005-03-01 00:00:00

abstract：:International guidelines emphasize the importance of LDL cholesterol (LDL-C) assay in the care and follow-up of patients with cardiovascular risk. Most studies and common practice use Friedewald's formula for LDL-C calculation. The accuracy of the result depends closely on the precision of the input parameters (total ...

journal_title：Annales de biologie clinique

authors： Bayer P,Veinberg F,Couderc R,Cherfils C,Cambillau M,Cosson C,Fradin S,Gillery P,Steinmetz J,Legrand A,Egloff M,Beucler I

更新日期：2005-01-01 00:00:00

abstract：:In 2003, for the scheme of the French national external quality assessment, Afssaps sent to medical laboratories a sample for which two analyses could be carried out: the electrophoresis of proteins and the characterization for monoclonal immunoglobulin. The purpose of this new approach was to make it possible to the ...

journal_title：Annales de biologie clinique

authors： Albarède S,Hattchouel JM,Guyard A,Nicolas A,Burg E,Daunizeau A,Pham BN

更新日期：2005-01-01 00:00:00

abstract：BACKGROUND:Systematic reviews and meta-analyses can help health-care professionals to base their decisions on the highest levels of evidence, and are therefore the cornerstone in practicing evidence-based medicine. In the field of diagnosis in general, and in laboratory medicine in particular, systematic reviews are sc...

journal_title：Annales de biologie clinique

authors： Watine J

更新日期：2004-11-01 00:00:00

abstract：:The aim of the study was to check if a slight and non visible hemolysis to naked eye such as that induced by blood coagulation could interfere in the immunonephelometric measurement of haptoglobin, and if this interference was dependent on the protein phenotype. Results confirmed that blood coagulation induced a non v...

journal_title：Annales de biologie clinique

authors： Guéye PM,Sall I,Lessinger JM,Férard G

更新日期：2004-11-01 00:00:00

abstract：:There is much variability in the manner individuals respond to drugs, such that the management of some drugs is problematic. In France, the incidence of hospital admissions related to adverse drug reactions is estimated to be 3.2 %, at an annual cost of over 300 millions euros. Genetic factors affecting the pharmacoki...

journal_title：Annales de biologie clinique

authors： Allorge D,Loriot MA

更新日期：2004-09-01 00:00:00

abstract：:Lipid profile is often performed on heparinized-plasma because nothing in particular is explained in the technical data sheet about anticoagulant and because few data (mainly with EDTA anticoagulant) are available in literature. In order to evaluate heparinized-plasma vs serum differences, 50 normo- or hyperlipidemic ...

journal_title：Annales de biologie clinique

authors： Sassolas A,Cheillan D,Drai J,Bondon PJ,Cartier R

更新日期：2004-09-01 00:00:00

abstract：:Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most frequent enzyme deficiency. It is a sex-linked genetic disease concerning mostly african, mediterranean and far-eastern populations. The main clinical expression is a hemolytic anemia which can be acute or chronic. During the neonatal period the disease m...

journal_title：Annales de biologie clinique

authors： Kaddari F,Sawadogo M,Sancho J,Lelong M,Jaby D,Paulin C,Nkana K,Cailliez M

更新日期：2004-07-01 00:00:00

abstract：:Among myeloproliferative disorders, Essential Thrombocythaemia is the most frequent and the one associated with the better prognosis. Although median survival is above 15 years, Essential Thrombocythaemia may be complicated by haemostatic life-threatening manifestations. Occurrence of thromboembolic or haemorrhagic ep...

journal_title：Annales de biologie clinique

authors： André-Kerneïs E,Gaussem P

更新日期：2004-05-01 00:00:00

abstract：:Two cases of newborn haemolytic disease because of ABO incompatibility are reported. In both cases, blood erythroblastosis exceeded 300 per 100 leukocytes at birth. Both newborns developed early jaundice and one presented with anaemia. Intensive phototherapy begun soon after birth was an effective treatment. The diagn...

journal_title：Annales de biologie clinique

authors： Palleau S,Le Tourneau C,Jrad I,Andreux JP,Gouin I,Siguret V

更新日期：2004-05-01 00:00:00

abstract：:Apolipoprotein B (apoB) is the major proteic component of LDL, VLDL and chylomicrons. Numerous polymorphisms of the apolipoprotein B gene have been described. Particularly, a polymorphism of insertion/deletion located in the coding part of the signal peptide of apoB, associated with modifications of lipid concentratio...

journal_title：Annales de biologie clinique

authors： Jemaa R,Mebazaa A,Fumeron F

更新日期：2004-03-01 00:00:00

abstract：:Micro- and nanospheres are tightly associated with the development of flow cytometry. They are indispensable tools to optimize diffraction and fluorescence signals as well as for fluorescence calibration and cellular purification (magnetic micro- and nanospheres). They are also usefull to evaluate phagocytosis and to ...

journal_title：Annales de biologie clinique

authors： Lizard G,Monier S,Prunet C,Duvillard L,Gambert P

更新日期：2004-01-01 00:00:00

abstract：:Immunofixation is widely used for monoclonal gammapathies identification and Bence Jones proteinuria research. In more of cases, interpretation is easy, but some difficulties persist. Five examples are presented and their causes are analysed; in our first case, seric immunofixation shows the problem generated by addit...

journal_title：Annales de biologie clinique

authors： Onraed B,Nguyen M,Lerche B,Hennache B

更新日期：2004-01-01 00:00:00

abstract：:In order to introduce the approach of HCV genotyping in our laboratory, a comparative study of 3 molecular and 1 serological methods, was conducted on 62 HCV RNA positive sera. The molecular genotyping methods target the 5'untranslated (UTR) region of the virus genome and are based on an amplification of the viral gen...

journal_title：Annales de biologie clinique

authors： Djebbi A,Sadraoui A,Triki H

更新日期：2003-11-01 00:00:00

abstract：:In assisted reproductive technology (ART), serum levels of several key hormones are used to evaluate ovarian follicular reserve and to monitor gonadotropin-stimulated follicle growth. Currently, serum estradiol, FSH, LH and inhibin B levels are examined and combined at the beginning of the menstrual cycle to evaluate ...

journal_title：Annales de biologie clinique

authors： Taieb J,Benattar C,Poüs C

更新日期：2003-09-01 00:00:00

abstract：:Hormonal production of the thyroid gland is constituted of thyroxine or T4 (80%) and triiodothyronine or T3 (20%). In the circulation, whole T4 originates from thyroid secretion but most of T3 (80%) is produced extrathyroidally from T4 deiodination. Conversion of T4 to T3 may be influenced by various conditions and ci...

journal_title：Annales de biologie clinique

authors： Sapin R,Schlienger JL

更新日期：2003-07-01 00:00:00

abstract：:Diagnosis of haematological malignancies is based on multiparametric analysis such as morphology, phenotype and genotype studies. Some entities are only defined by one of these approach. Flow-cytometry (FCM) is useful to determined the normal counterpart of the tumoral process and its differentiation status within the...

journal_title：Annales de biologie clinique

authors： Drénou B,Fardel O,Fauchet R,Amiot L

更新日期：2002-11-01 00:00:00

abstract：:Our data suggest that the hyperhomocysteinemia and/or increased plasma level of lipoprotein Lp(a) are risk factors for coronary heart disease. We investigated 178 patients who underwent complete cardiac examination comprising coronary angiography and biological analysis (CT, HDL-c, LDL-c, TG, and apoAI, apoB, homocyst...

journal_title：Annales de biologie clinique

authors： Laraqui A,Bennouar N,Meggouh F,Allami A,El Kadiri N,Benkouka F,Azeddoug H,El Haitem N,Benomar A,Fellat S,Benomar M

更新日期：2002-09-01 00:00:00

abstract：:Cytomegalovirus infection is a major problem for transplant recipients. It has been recently shown that the rate of increase in viral load correlate with CMV disease in transplant recipients. The risk is very high in persons undergoing bone marrow transplantation and in some cases preemptive therapy is used as soon as...

journal_title：Annales de biologie clinique

authors： Schvoerer E,Stoll-Keller F

更新日期：2002-07-01 00:00:00

abstract：:To validate the use of the i-STAT portable point-of-care instrument, performance checks have been conducted to verify its accuracy for PCO2/PO2 (tonometry) and precision for pH/PCO2/PO2 (control solutions). Results obtained with the i-STAT (pH/PCO2/PO2/Hct) were also compared to those provided by the Bayer Rapidlab 86...

journal_title：Annales de biologie clinique

authors： Feuillu A,Morel I,Mollard JF

更新日期：2002-03-01 00:00:00

abstract：:A reversed-phase high-performance liquid chromatographic assay for the determination of the HIV protease inhibitors amprenavir (Agenerase) and indinavir (Crixivan) in human plasma is described, using a mobile phase consisting of 0.50 M phosphate buffer (adjusted to pH 5,5) - Milli-Q water - acetonitrile (120: 1,080: 8...

journal_title：Annales de biologie clinique

authors： Laussine S,Roux A,Delhotal-Landes B,Flouvat B

更新日期：2001-03-01 00:00:00

abstract：:Schistocytes result from red cell fragmentation. The identification of the schistocytes is critical for appropriate management of the patients. We report the results of a survey about the determination of schistocytes. The analysis of the answers of 24 well-trained biologists points out the different approaches used t...

journal_title：Annales de biologie clinique

authors： Lesesve JF,Salignac S,Lecompte T

更新日期：2001-01-01 00:00:00

abstract：:Macroprolactin is a complex of prolactin with immunoglobulins (IgG) that has limited or no biological activity in vivo. Immunoassays for prolactin have variable reactivity with macroprolactin. Therefore the presence of macroprolactin should be considered in the differential diagnosis of hyperprolactinemia. We compared...

journal_title：Annales de biologie clinique

authors： Sapin R,Gasser F,Fischbach E,Grucker D

更新日期：2000-11-01 00:00:00

abstract：:The clinical use of the direct inhibitors of thrombin requires a reliable test to monitor the treatment and to predict the hemorragic risk. The activated partial thromboplastin time (APTT) is the most common test used to monitor treatment with unfractionated heparin. Thus APTT has been first chosen to follow patients ...

journal_title：Annales de biologie clinique

authors： Kher A,Gouin I,Samama MM

更新日期：2000-09-01 00:00:00

abstract：:Myelodysplastic syndromes (MDS) are clonal disorders of pluripotent hematopoietic stem cells. MDS occur predominantly over the age of 60 years. The diagnosis of MDS is based on the examination of both blood films and bone marrow aspirate. Diseases such as vitamin B12 and/or folate deficiency, or cytotoxic therapy lead...

journal_title：Annales de biologie clinique

authors： Garandeau C,Pautas E,Andreux M,Andreux J,Gaussem P,Siguret V

更新日期：2000-07-01 00:00:00

abstract：:http://biotribune.com/ Biotribune.com ou la biologie medicale sur internet. Ce site est concu comme un portail sur la biologie clinique. Accueil d'internautes sur une page comportant, entre autres, les rubriques : actualite, evenements, revue des connaissances, rubrique juridique et petites annonces. Biotribune propos...

journal_title：Annales de biologie clinique

authors： Couderc R

更新日期：2000-07-01 00:00:00

abstract：:Caveolae are small pockets or invaginations localized at the plasma membrane. They are enriched in glycosphingolipids, cholesterol, sphingomyelin and lipid-enchored membrane proteins, and they are characterized by a light buoyant density and resistance to solubilization by Triton X-100 at 4 C. Caveolins are the princi...

journal_title：Annales de biologie clinique

authors： Roch-Arveiller M,Couderc R

更新日期：2000-03-01 00:00:00

abstract：:We retrospectively evaluated measurement data and clinical relevance of autoantibodies to gangliosides in peripheral neuropathies (PN). The IgG and IgM antiganglioside autoantibodies were determined by our own immunodot-blot assay on membrane and by enzyme-linked immunosorbent assay (Elisa) in sera of 1,342 patients w...

journal_title：Annales de biologie clinique

authors： Caudie C,Vial C,Petiot P,Bancel J,Later R,Gonnaud PM

更新日期：1999-09-01 00:00:00

abstract：:A group of genetic disorders of the red cell, appearing as congenital haemolytic anaemias, were first described on morphological bases, due mainly to the presence of stomatocytes. It turned out, however, that the alterations of the shape could widely vary, and even be absent, thereby questionning the classification on...

journal_title：Annales de biologie clinique

authors： Delaunay J

更新日期：1999-07-01 00:00:00

abstract：:Depression is a pathology frequently observed in general medicine (10%). Treatment of depression makes great use of drugs from different pharmacological classes. The optimal posology is difficult to establish, and clinicians prefer to avoid side effects by prescribing low regimen. This study is related to an evaluatio...

journal_title：Annales de biologie clinique

authors： Charlier C,Ansseau M,Pinto E,Andrien F,Plomteux G

更新日期：1999-07-01 00:00:00

abstract：:In this review, after a brief summary of the spermatogenesis process, we present some genetic causes of male infertility at chromosome and gene level, known at present. Even though knowledge has greatly increased, in 15% of cases, we still do not know the infertility mechanism. The genotype-phenotype relationship rema...

journal_title：Annales de biologie clinique

authors： Seifer I,Fellous M,Bignon Y

更新日期：1999-05-01 00:00:00

abstract：:The recent development of specific and sensitive biochemical markers reflecting the overall rate of bone formation and bone resorption, has markedly improved the non invasive assessment of bone turnover in various metabolic bone diseases, especially for osteoporosis. The immunoassay of human osteocalcin recognizing th...

journal_title：Annales de biologie clinique

authors： Garnero P,Delmas PD

更新日期：1999-03-01 00:00:00

abstract：:Cardiac troponin T (troponin Tc) of second generation has been measured on the Boehringer Elecsys 2010 analyzer. Cardiac specificity has been studied in patients presenting a rhabdomyolysis syndrome and the results compared with those obtained for cardiac troponin I (troponin Ic) measured on the Dade-Behring Stratus a...

journal_title：Annales de biologie clinique

authors： Cauliez B,Hue G,Lavoinne A

更新日期：1998-11-01 00:00:00

abstract：:HTLV genomic and antigenic features, replication way as well as associated pathology are recalled herein. The epidemiologic angle and the different transmission ways are also related. HTLV infection diagnostic implements are detailed: screening and specially confirmatory tests are brought to light with the help of con...

journal_title：Annales de biologie clinique

authors： Maniez-Montreuil M

更新日期：1998-09-01 00:00:00

abstract：:The presence of the R506Q mutation of the factor V gene is associated with an increased risk of thromboembolism, particularly during pregnancy. Recently, its involvement in the development of obstetrical complications, such as preeclampsia and fetal losses, has been evoked. The resulting factor VQ506 (factor V Leiden)...

journal_title：Annales de biologie clinique

authors： Magdelaine A,Soubrier F,Berkane N,Uzan S,Verdy E

更新日期：1998-07-01 00:00:00

abstract：:In Alzheimer's disease, cortical areas of affected patients are invaded by extracellular proteinous deposits called senile plaques, the main component of which is called amyloid beta-peptide or A beta. This peptide derives from the proteolytic attack of a precursor, the beta-amyloid precursor protein, by two enzymes c...

journal_title：Annales de biologie clinique

authors： Marambaud P,Chevallier N,Lopez-Perez E,Drouot C,Vizzanova J,Fulcrand P,Martinez J,Wilk S,Checler F

更新日期：1998-05-01 00:00:00

abstract：:Various coagulation abnormalities were reported in HIV-infected patients. Cases of severe thrombocytopenia were first observed in contaminated homosexual males, as well as prolonged APTT due to the presence of lupus-like anticoagulant with a frequency in the range 8 to 70% of the studied patients. Even if lupus antico...

journal_title：Annales de biologie clinique

authors： Toulon P

更新日期：1998-03-01 00:00:00

abstract：:Community viral bronchopneumonias are frequent, mainly in children, and can be associated to all respiratory viruses: influenza- and parainfluenzavirus, respiratory syncytial virus, adenovirus, rhinovirus. The diagnostic method which proves viral infection of the respiratory tissues is selected as the direct detection...

journal_title：Annales de biologie clinique

authors： Freymuth F,Vabret A,Galateau F,Brouard J,Eugene G,Petitjean J,Gennetay E

更新日期：1998-01-01 00:00:00

abstract：:The GSH-400 method (Oxis International SA) performed in acid supernatant, provides an assay of reduced glutathione (L-gamma-glutamyl-L-cysteinylglycine) (GSH) in biological samples by a two step chemical reaction followed by a spectrophotometric detection. We improved the analytical performances of the assay by using ...

journal_title：Annales de biologie clinique

authors： Boutolleau D,Lefévre G,Etienne J

更新日期：1997-11-01 00:00:00